The unique information on ability of some amphibians regenerate severely damaged tissues though de-differentiation re-differentiation is encoded in genetic makeup of amphibian oocytes. Using electroporation, it is possible to activate natural reprogramming potential of living Xenopus Laevis frog oocytes and pass it to donor cells placed with eggs in one elctroporation chamber. We demonstrated that co-electroporation of mature oocytes with suspension of different normal and cancerous human cells Lines such as: bone marrow stromal cells , foreskin fibroblasts, pre-adipocytes, CD4+T-lymphocytes, cheek cells, cervical carcinoma (HeLa) cells and breast adenocarcinoma (MCF-7) cells reprograms donor cells into iPSc- like cells which form colonies on irradiated MEF feeders. Obtained iPSc-like cells resemble human embryonic stem cells in colony morphology, and expression of stem cell associated transcription factors: Oct3/4, Nanog, SOX-2, Rex-1, TRA-1-60, SSEA-1 and SSEA-4. Efficacy of reprogramming (calculated for CD4+lymphocytes only) was 23.4±3.5%. Advantageously, new method obviates use of retroviral or lentiviral gene delivery vectors and other “non-parental” reprogramming approaches and may hold great promise for the use in rapid and inexpensive production of human autologous stem cells.